Coding

Part:BBa_M36670:Design

Designed by: Richard Fuisz   Group: Stanford BIOE44 - S11   (2014-10-24)


Bxb1 Recombinase + NLS on N terminus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1105
    Illegal NgoMIV site found at 1192
    Illegal AgeI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


Design Notes

Added a NLS to Bxb1 integrase to help promote accurate localization to the nucleus


Source

https://parts.igem.org/Part:BBa_K907000 For Bxb1 sequence

References